Electrophysiological characterisation of iPSC-derived human β-like cells and an SLC30A8 disease model.

Manon Jaffredo Nicole A J KrentzBenoite ChamponClaire E Duff Sameena Nawaz Nicola L Beer Christian Honoré Anne Clark Patrik Rorsman Jochen Lang Anna L Gloyn Matthieu Raoux Benoit Hastoy

Published in: bioRxiv : the preprint server for biology (2023)

Our findings validate the use of intra- and extra-cellular electrophysiology to assess and monitor the functions of BLCs. Our approach opens the perspective of using MEAs to live-monitor the differentiation quality of iPSC-derived BLCs and to determine the functional consequences of diabetes-associated variants.

Keyphrases

induced pluripotent stem cells
endothelial cells
type diabetes
cardiovascular disease
copy number
glycemic control
quality improvement
gene expression
metabolic syndrome
adipose tissue
dna methylation
skeletal muscle
genome wide