Effect of prevalent polychlorinated biphenyls (PCBs) food contaminant on the MCF7, LNCap and MDA-MB-231 cell lines viability and PON1 gene expression level: proposed model of binding.
Fatemeh YazdiShahram ShoeibiMohammad-Hossein YazdiAkram EidiPublished in: Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences (2021)
Current results showed that despite of hormone non-responsive cells the PCBs have a significant positive effect on hormone-responsive cell. Therefore, and regarding to the existence of PCBs contamination in food there should be serious concern about their impact on the prevalence of different malignancies which certainly should result in a standard limit for this material. This study aimed to investigate the direct effects of prevalent PCBs in food in hormone-responsive and non-responsive cell lines. Cell lines were treated with serial concentrations of PCBs and cell viability assessment was performed using MTT assay. The expression level of PON1 was evaluated using real-time PCR. Molecular docking was performed to determine the binding mechanism and predicted binding energies of PBCs compounds to the AhR receptor. PCBs contamination in food there should be serious concern about their impact on the prevalence of different malignancies which certainly should result in a standard limit for this material.
Keyphrases
- molecular docking
- human health
- gene expression
- cancer therapy
- risk assessment
- binding protein
- risk factors
- real time pcr
- breast cancer cells
- induced apoptosis
- drinking water
- dna binding
- dna methylation
- high throughput
- molecular dynamics simulations
- stem cells
- health risk
- signaling pathway
- mesenchymal stem cells
- oxidative stress
- density functional theory
- molecular dynamics
- heavy metals
- endoplasmic reticulum stress