Small RNA sequencing in hypoxic naked mole-rat hearts suggests microRNA regulation of RNA- and translation-related processes.
W Aline Ingelson-FilpulaHang ChengLiam EatonMatthew E PamenterKenneth B StoreyPublished in: FEBS letters (2022)
The naked mole-rat (Heterocephalus glaber) regularly endures intermittent periods of hypoxia in its burrows, surviving in part due to metabolic rate depression (MRD)-a strategy of conserving cellular resources by downregulating nonessential gene expression and reorganizing cellular processes. miRNA are short, noncoding RNAs already implicated for their roles in numerous models of extreme environmental stress; given their rapid, reversible nature, they are ideal for implementing MRD. We performed small RNA sequencing on cardiac tissue from normoxic versus 24 h hypoxic naked mole-rats, and used bioinformatics to predict 18 miRNAs which may be differentially regulated during hypoxia. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway mapping further suggest these miRNAs play roles in largely translation-related functions, including RNA processing and catabolism.
Keyphrases
- gene expression
- single cell
- genome wide
- oxidative stress
- dna methylation
- endothelial cells
- genome wide identification
- depressive symptoms
- transcription factor
- squamous cell carcinoma
- high resolution
- heart failure
- physical activity
- sleep quality
- mass spectrometry
- stress induced
- atrial fibrillation
- drug induced
- heat stress
- rectal cancer
- quantum dots