The 6-kilodalton peptide 1 in plant viruses of the family Potyviridae is a viroporin.
Mengzhu ChaiLei LiYong LiYingshuai YangYuting WangXue JiangYameng LuanLongjiang FanHongguang CuiAiming WangWen-Sheng XiangXiaoyun WuXiaofei ChengPublished in: Proceedings of the National Academy of Sciences of the United States of America (2024)
Potyviridae, the largest family of plant RNA viruses, includes many important pathogens that significantly reduce the yields of many crops worldwide. In this study, we report that the 6-kilodalton peptide 1 (6K1), one of the least characterized potyviral proteins, is an endoplasmic reticulum-localized protein. AI-assisted structure modeling and biochemical assays suggest that 6K1 forms pentamers with a central hydrophobic tunnel, can increase the cell membrane permeability of Escherichia coli and Nicotiana benthamiana , and can conduct potassium in Saccharomyces cerevisiae . An infectivity assay showed that viral proliferation is inhibited by mutations that affect 6K1 multimerization. Moreover, the 6K1 or its homologous 7K proteins from other viruses of the Potyviridae family also have the ability to increase cell membrane permeability and transmembrane potassium conductance. Taken together, these data reveal that 6K1 and its homologous 7K proteins function as viroporins in viral infected cells.
Keyphrases
- saccharomyces cerevisiae
- endoplasmic reticulum
- escherichia coli
- sars cov
- induced apoptosis
- dna repair
- high throughput
- dna damage
- endothelial cells
- signaling pathway
- genetic diversity
- single cell
- oxidative stress
- dna methylation
- big data
- gene expression
- cystic fibrosis
- endoplasmic reticulum stress
- multidrug resistant
- ionic liquid
- antimicrobial resistance
- cell wall
- small molecule
- klebsiella pneumoniae
- biofilm formation
- machine learning
- pseudomonas aeruginosa
- amino acid