Transcriptional Profile of Kidney from Type 2 Diabetic db/db Mice.
Haojun ZhangTingting ZhaoZhiguo LiMeihua YanHailing ZhaoBin ZhuPing LiPublished in: Journal of diabetes research (2017)
Diabetic nephropathy (DN), a common diabetic microvascular complication, is characterized by progressive glomerular sclerosis and tubulointerstitial fibrosis. However, the underlying mechanisms involved in DN remain to be elucidated. We explored changes in the transcriptional profile in spontaneous type 2 diabetic db/db mice by using the cDNA microarray. Compared with control db/m mice, the db/db mice exhibited marked increases in body weight, kidney weight, and urinary albumin excretion. Renal histological analysis revealed mesangial expansion and thickness of the basement membrane in the kidney of the db/db mice. A total of 355 differentially expressed genes (DEGs) were identified by microarray analysis. Pathway enrichment analysis suggested that biological oxidation, bile acid metabolism, and steroid hormone synthesis were the 3 major significant pathways. The top 10 hub genes were selected from the constructed PPI network of DEGs, including Ccnb2 and Nr1i2, which remained largely unclear in DN. We believe that our study can help elucidate the molecular mechanisms underlying DN.
Keyphrases
- diabetic nephropathy
- high fat diet induced
- body weight
- type diabetes
- gene expression
- body mass index
- adipose tissue
- bioinformatics analysis
- wild type
- physical activity
- wound healing
- weight loss
- metabolic syndrome
- wastewater treatment
- insulin resistance
- endothelial cells
- hydrogen peroxide
- weight gain
- genome wide identification
- heat shock protein
- heat shock