Real-time visualization of sulfatase in living cells and in vivo with a ratiometric AIE fluorescent probe.

Different from the traditional enzymatic hydrolysis strategy, we rationally developed a ratiometric fluorescence probe DQMT-OH with AIE characteristics for sulfatase detection utilizing the "Lock-Key" strategy. It can be successfully used to monitor sulfatase in living cells and in vivo through different fluorescent channels with good cell permeability and low cytotoxicity.