WISP-3 Stimulates VEGF-C-Dependent Lymphangiogenesis in Human Chondrosarcoma Cells by Inhibiting miR-196a-3p Synthesis.
Chih-Yang LinShih-Wei Wang LJeng-Hung GuoHuai-Ching TaiWen-Chun SunCheng-Ta LaiChen-Yu YangShih-Chia LiuYi-Chin FongChih-Hsin TangPublished in: Biomedicines (2021)
Chondrosarcoma is a malignant bone tumor with high metastatic potential. Lymphangiogenesis is a critical biological step in cancer metastasis. WNT1-inducible signaling pathway protein 3 (WISP-3) regulates angiogenesis and facilitates chondrosarcoma metastasis, but the role of WISP-3 in chondrosarcoma lymphangiogenesis is unclear. In this study, incubation of chondrosarcoma cells with WISP-3 increased the production of VEGF-C, an important lymphangiogenic factor. Conditioned medium from WISP-3-treated chondrosarcoma cells significantly enhanced lymphatic endothelial cell tube formation. WISP-3-induced stimulation of VEGF-C-dependent lymphangiogenesis inhibited miR-196a-3p synthesis in the ERK, JNK, and p38 signaling pathways. This evidence suggests that the WISP-3/VEGF-C axis is worth targeting in the treatment of lymphangiogenesis in human chondrosarcoma.
Keyphrases
- endothelial cells
- signaling pathway
- induced apoptosis
- high glucose
- vascular endothelial growth factor
- pi k akt
- cell cycle arrest
- endoplasmic reticulum stress
- stem cells
- epithelial mesenchymal transition
- cell proliferation
- oxidative stress
- cell death
- squamous cell carcinoma
- papillary thyroid
- lymph node
- young adults
- climate change
- newly diagnosed
- diabetic rats