Stable contacts of naïve CD4 T cells with migratory dendritic cells are ICAM-1-dependent but dispensable for proliferation in vivo.
Stav KozlovskiOfir AtrakchiSara W FeigelsonZiv ShulmanRonen AlonPublished in: Cell adhesion & migration (2020)
It is unclear if naïve T cells require dendritic cell ICAMs to proliferate inside lymph nodes. To check if and when CD4 lymphocytes use ICAMs on migratory DCs, wild-type and ICAM-1 and 2 double knock out bone marrow-derived DCs pulsed with saturating levels of an OT-II transgene-specific ovalbumin-derived peptide were co-transferred into skin-draining lymph nodes. Intravital imaging of OT-II lymphocytes entering these lymph nodes revealed that ICAM-1 and -2 deficient migratory DCs formed fewer stable conjugates with OT-II lymphocytes but promoted normal T cell proliferation. DC ICAMs were also not required for unstable TCR-dependent lymphocyte arrests on antigen presenting migratory DCs. Thus, rare antigen-stimulated ICAM-stabilized T-DC conjugates are dispensable for CD4 lymphocyte proliferation inside lymph nodes.
Keyphrases
- lymph node
- dendritic cells
- peripheral blood
- regulatory t cells
- wild type
- sentinel lymph node
- cell proliferation
- neoadjuvant chemotherapy
- immune response
- signaling pathway
- high resolution
- mesenchymal stem cells
- nk cells
- case report
- cell cycle
- radiation therapy
- squamous cell carcinoma
- single cell
- drug delivery
- cardiac arrest
- early stage
- mass spectrometry
- cardiopulmonary resuscitation
- photodynamic therapy