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Hierarchical Quatsome-RGD Nanoarchitectonic Surfaces for Enhanced Integrin-Mediated Cell Adhesion.

Marc Martínez-MiguelMiquel Castellote-BorrellMariana KöberAdriana R KyvikJudit Tomsen-MeleroGuillem Vargas-NadalJose MuñozDaniel PulidoEdgar Cristóbal-LecinaSolène PassemardMiriam RoyoMarta Mas-TorrentJaume VecianaMarina Inés GiannottiJudith GuaschNora VentosaImma Ratera
Published in: ACS applied materials & interfaces (2022)
The synthesis and study of the tripeptide Arg-Gly-Asp (RGD), the binding site of different extracellular matrix proteins, e.g., fibronectin and vitronectin, has allowed the production of a wide range of cell adhesive surfaces. Although the surface density and spacing of the RGD peptide at the nanoscale have already shown a significant influence on cell adhesion, the impact of its hierarchical nanostructure is still rather unexplored. Accordingly, a versatile colloidal system named quatsomes, based on fluid nanovesicles formed by the self-assembling of cholesterol and surfactant molecules, has been devised as a novel template to achieve hierarchical nanostructures of the RGD peptide. To this end, RGD was anchored on the vesicle's fluid membrane of quatsomes, and the RGD-functionalized nanovesicles were covalently anchored to planar gold surfaces, forming a state of quasi-suspension, through a long poly(ethylene glycol) (PEG) chain with a thiol termination. An underlying self-assembled monolayer (SAM) of a shorter PEG was introduced for vesicle stabilization and to avoid unspecific cell adhesion. In comparison with substrates featuring a homogeneous distribution of RGD peptides, the resulting hierarchical nanoarchitectonic dramatically enhanced cell adhesion, despite lower overall RGD molecules on the surface. The new versatile platform was thoroughly characterized using a multitechnique approach, proving its enhanced performance. These findings open new methods for the hierarchical immobilization of biomolecules on surfaces using quatsomes as a robust and novel tissue engineering strategy.
Keyphrases
  • cell adhesion
  • extracellular matrix
  • tissue engineering
  • biofilm formation
  • single cell
  • mass spectrometry
  • stem cells
  • high resolution
  • high throughput
  • pseudomonas aeruginosa
  • high speed