In this letter, by measuring resistivity and magnetization with magnetic field H rotated in ab plane and current I along c axis, we studied the magnetic and electronic structure symmetry of frustrated topological bilayer Kagome ferromagnet Fe3Sn2. We observed that the curves of the resistivity and magnetization both showed broken two-fold symmetry from 5 K to 380 K. The further analysis indicates that there is a close causality between the spin arrangement and the electronic states in Fe3Sn2 even above room temperature. These phenomena are closely related to the change in spin-orbit coupling under the magnetic field. Our experimental results suggest that Fe3Sn2 is an ideal platform to study the influence of spin arrangement on electronic state in topological materials and can also be used to design next generation magnetic devices by modulating spin-orbit coupling at external magnetic field.