In Vitro and In Vivo Anti-Inflammatory Properties of the Hydroethanolic Extract of the Roots of Vernonia guineensis (Asteraceae).
William Yousseu NanaJustin Rodrigue Billong MimbAlbert Donatien AtsamoEric Gonzal TsafackStephanie Flore Djuichou NguemnangZenab Linda Fagni NjoyaVanessa Mba Matah MartheYacine Karelle Madjo KouamMbiantcha MariusGilbert AteufackPublished in: International journal of inflammation (2023)
In traditional Cameroonian medicine, to relieve many inflammations, parts of Vernonia guineensis , are very widely used. This study considered the evaluation of acute toxicity and anti-inflammatory properties of the hydroethanolic extract of the roots of Vernonia guineensis . In an acute toxicity study, 250, 2500, and 5000 mg/kg were administered orally to mice in a single dose, and general behavior, adverse effects, and mortality were monitored. In vitro and in vivo anti-inflammatory tests were performed, and then histological, serum, hematological, and oxidative stress parameters have been evaluated. In an acute toxicity, all groups revealed neither mortality nor any significant alteration in behavior; only drowsiness, sedation, and lethargy were observed at 5000 mg/kg. For in vitro tests, the extract inhibited anti-inflammatory activity. In the formalin test, at 250 mg/kg, the extract inhibited edema with a percentage of 56.41% (4 th hour) in an acute treatment and 74.44% (10 th day). Joint edema was reduced by 67.24% (24 th hour) in a single treatment and by 74.25% (7 th day) in repeated treatment. The extract caused an increase in red blood cell, hemoglobin, and serum protein levels and reduced the white blood cells as well as the activities of alkaline phosphatase and alanine aminotransferase. The extract modulated oxidative stress parameters in the brain, spinal cord, liver, and kidneys. The extract protected the joint by reducing the bone and cartilage erosion. The present work highlights the anti-inflammatory, antioxidant, and antianemic properties of the hydroethanolic extract of the roots of Vernonia guineensis , which supports its empirical use in traditional medicine for the treatment of inflammatory pathologies.
Keyphrases
- anti inflammatory
- oxidative stress
- induced apoptosis
- spinal cord
- ischemia reperfusion injury
- red blood cell
- dna damage
- respiratory failure
- diabetic rats
- spinal cord injury
- coronary artery disease
- combination therapy
- skeletal muscle
- heat stress
- intensive care unit
- bone mineral density
- drug induced
- aortic dissection
- multiple sclerosis
- brain injury
- adipose tissue
- subarachnoid hemorrhage
- cardiovascular events
- mechanical ventilation
- heat shock
- neuropathic pain
- body composition
- acute respiratory distress syndrome
- small molecule
- high fat diet induced
- protein protein
- hepatitis b virus
- insulin resistance