Differentiation of 2-hydroxyglutarate enantiomers and its lactones by gas chromatography/electron ionization tandem mass spectrometry.
Shinji K StrainMorris D GrovesMark R EmmettPublished in: Rapid communications in mass spectrometry : RCM (2020)
Unique EI fragmentation pathways for both 2-hg and the 2-hg lactone have been described. Subsequently, the GC/MS/MS assay presented herein has significant potential as a novel clinical assay as it separates and detects both 2-hg enantiomers and the 2-hg lactone enantiomers, a capability which has not been previously demonstrated by any other assay to date.
Keyphrases
- gas chromatography
- tandem mass spectrometry
- ultra high performance liquid chromatography
- mass spectrometry
- high performance liquid chromatography
- fluorescent probe
- liquid chromatography
- high resolution mass spectrometry
- capillary electrophoresis
- high throughput
- simultaneous determination
- aqueous solution
- gas chromatography mass spectrometry
- ms ms
- solid phase extraction
- living cells
- high resolution
- liquid chromatography tandem mass spectrometry
- risk assessment
- human health