Selecting the Fluorescent Protein for In Vivo Imaging Experiments.

Many fluorescent proteins are available nowadays suitable for in vivo imaging experiments. Each fluorescent protein has unique biophysical properties, such as emission and excitation spectra, quantum yield, oligomerization state, pH sensitivity, fluorescence lifetime, and stability within the cellular environment. Even a small variation in fluorescent protein properties might result in significant differences in the experimental outcomes. This chapter discusses the aspects that need to be considered while selecting the fluorescent proteins for in vivo imaging experiment.