Direct screening of enzyme activity using infrared matrix-assisted laser desorption electrospray ionization.

Milad NazariMåns EkelöfSitora KhodjaniyazovaNathaniel L ElsenJon D WilliamsDavid C Muddiman

Published in: Rapid communications in mass spectrometry : RCM (2018)

The enzymatic activity of IDH1 was screened by directly analyzing the reaction content from the buffer in well plates with no sample cleanup steps. This proof-of-concept study demonstrates the robustness of IR-MALDESI for direct analysis of enzymatic reactions from biological buffers with no sample cleanup and its immense potential for HTS applications.

Keyphrases

hydrogen peroxide
solid phase extraction
low grade
high speed
nitric oxide
human health
risk assessment
mass spectrometry
climate change
simultaneous determination