TREC/KREC Levels in Young COVID-19 Patients.
Maryam B KhadzhievaEkaterina V KalininaSergey S LarinDaria A SviridovaAlesya S GrachevaJulia V ChursinovaVadim A StepanovIvan V RedkinLyudmila S AvdeikinaAlexander G RumyantsevArtem N KuzovlevLyubov E SalnikovaPublished in: Diagnostics (Basel, Switzerland) (2021)
COVID-19 patients with acute respiratory distress syndrome (ARDS) have an immune imbalance when systemic inflammation and dysfunction of circulating T and B cells lead to a more severe disease. Using TREC/KREC analysis, we studied the level of mature naive T and B cells in peripheral blood of COVID-19 patients and its relationship with clinical and laboratory data. TREC/KREC analysis was performed by multiplex real-time quantitative PCR on a sample of 36 patients aged 45 years or younger. The reduced TREC/KREC level was observed in ARDS patients compared with non-ARDS patients, and similar results were found for the deceased patients. During days 6 to 20 of hospitalization, a higher neutrophil-to-lymphocyte ratio (NLR) was detected in ARDS patients compared with non-ARDS patients. TREC/KREC negatively correlated with NLR; the highest correlation was recorded for TREC per 100,000 cells with the coefficient of determination R2 = 0.527. Thus, TREC/KREC analysis is a potential prognostic marker for assessing the severity and outcome in COVID-19.
Keyphrases
- acute respiratory distress syndrome
- end stage renal disease
- newly diagnosed
- chronic kidney disease
- ejection fraction
- oxidative stress
- mechanical ventilation
- machine learning
- intensive care unit
- patient reported outcomes
- high resolution
- high throughput
- computed tomography
- artificial intelligence
- signaling pathway
- big data
- data analysis
- pi k akt