Synthetic diets containing a single polysaccharide disrupt gut microbial community structure and microbial interaction networks in the American cockroach.

Achieving and maintaining a healthy gut microbiome has numerous benefits for the host. Host diet plays a key role in shaping the gut microbial community, and understanding how diet composition influences gut microbiome structure and stability is key to developing effective interventions to treat gut microbiome dysbiosis. We use the American cockroach ( Periplaneta americana ) as a model system to dissect the response of gut microbes to host diet modification. Here, we designed synthetic diets from lab-grade, purified ingredients to identify how the cockroach gut community responds to different carbohydrate components (chitin, methylcellulose, microcrystalline cellulose, pectin, starch, xylan) in otherwise balanced diets. Using 16S rRNA gene sequencing, we show that synthetic diets produce replicable shifts in the cockroach gut community diversity and phylogenetic composition, with xylan-fed insects displaying the largest alterations. Comparison with cockroaches fed whole-food diets reveal that, rather than introducing new microbes, synthetic diets alter microbiome composition by inducing blooms among taxa present basally within the cockroach gut community. Synthetic diets are also associated with less-robust, more fragmentary microbial co-occurrence networks compared to cockroaches fed whole-food diets. Our results highlight the utility of lab-grade artificial diets in microbiome research and shed light on how purified polysaccharides may exert more influence over a stable gut community to generate noticeable change than whole food-derived fibers.