Single molecule based SNP detection using designed DNA carriers and solid-state nanopores.

Jinglin KongJinbo ZhuUlrich Felix Keyser

Published in: Chemical communications (Cambridge, England) (2017)

Single nucleotide polymorphisms (SNPs) play a crucial role as molecular markers in medical and diagnostic application. We demonstrate a nanopore based method for SNP detection at the single molecule level. Designed DNA carriers are used to distinguish DNA strands containing only one single base difference and follow strand displacement kinetics.

Keyphrases

single molecule
solid state
genome wide
atomic force microscopy
living cells
loop mediated isothermal amplification
healthcare
real time pcr
dna methylation
circulating tumor
high density
quantum dots