Preventative Effects of Vitamin E on Testicular Damage and Sperm Parameters in the First-Generation Mice Pups due to Pre- and Postnatal Mancozeb Exposure.

The present study aimed to evaluate the effects of vitamin E on mancozeb-induced testis damage of the first-generation pups during intrauterine and lactating periods. Two groups of pregnant NMRI mice received 500 mg/kg mancozeb (MNZ) as MNZ group and 200 mg/kg vitamin E as MNZ+vit.E group before receiving MNZ. In addition, a vehicle and a control group were designed every other day in gestation and lactation periods. The male pups from each group were maintained until adulthood (8-10 W). The left testes and epididymides were removed following the sacrifice of the pups. Then, they were weighed, and sperm parameters including number, viability, motility, and morphology and testis structure were evaluated. A significant decrease occurred in sperm parameters of the mancozeb-treated pups compared to the control and vehicle groups. Treatment with vitamin E reversed the deleterious effects of MNZ to a nearly normal condition. Testis parameters including the weight, gonadosomatic index, seminiferous tubule diameters, and Johnsen's score, as well as the number of germ cells such as spermatogonia, spermatocyte, spermatid, and Sertoli, decreased significantly in the MNZ group, compared to the amount in the control and vehicle groups. Interestingly, the treatment with vitamin E was reversed in most of these parameters. Based on the results, the exposure of pups to mancozeb during pregnancy and lactating periods negatively affects the reproductive system of male pups. However, the coadministration of vitamin E could prevent the deleterious effects of mancozeb on sperm and testis parameters.