Panspecies molecular assays detect viral pathogens missed by real-time PCR/reverse-transcriptase PCR among pneumonia patients, Sarawak, Malaysia.

Jane K FieldhouseEmily S BaileyTeck-Hock TohKing-Ching HiiKerry A MallinsonJakie TingJohn A LednickyAntoinette BeritaTham Thi NguyenDiego GalanSon T ThanSee-Chang WongToh-Mee WongPatrick J BlairGregory C Gray

Published in: Tropical diseases, travel medicine and vaccines (2020)

The observed differences in the two modes of amplification suggest that where a problem with sensitivity is suspected, real-time assay results might be supplemented with panspecies conventional PCR/RT-PCR assays.

Keyphrases

real time pcr
high throughput
end stage renal disease
ejection fraction
chronic kidney disease
sars cov
prognostic factors
pulmonary embolism
patient reported outcomes
multidrug resistant
gram negative
single cell
patient reported