Ensuring quality in 17OHP mass spectrometry measurement: an international study assessing isomeric steroid interference.
Chung Shun HoKirsten HoadBrian R CookeTrisha AndersenPeter GrahamSjoerd A A van den BergMichaela F HartmannClara W S LoTze Ping LohYolanda B de RijkeBertrand D van ZelstStefan A WudyRosita ZakariaRonda F GreavesPublished in: Clinical chemistry and laboratory medicine (2023)
Most participating laboratories demonstrated their ability to reliably measure 17OHP in the presence of the four clinically relevant isomeric steroids. The performance of the 12 (32 %) laboratories that did not engage in this activity remains unclear. We recommend that all laboratories offering LC-MS/MS analysis of 17OHP in serum, plasma, or dried bloodspots determine that the isomeric steroids are appropriately separated.