Impact of Sustained Fructose Consumption on Gastrointestinal Function and Health in Wistar Rats: Glycoxidative Stress, Impaired Protein Digestion, and Shifted Fecal Microbiota.
Guadalupe Sánchez-TerrónRemigio MartínezJorge RuizCarolina LunaMarta Suely MadrugaPublished in: Journal of agricultural and food chemistry (2023)
The gastrointestinal tract (GIT) is the target of assorted pathological conditions, and dietary components are known to affect its functionality and health. In previous in vitro studies, we observed that reducing sugars induced protein glycoxidation and impaired protein digestibility. To gain further insights into the pathophysiological effects of dietary sugars, Wistar rats were provided with a 30% (w/v) fructose water solution for 10 weeks. Upon slaughter, in vivo protein digestibility was assessed, and the entire GIT (digests and tissues) was analyzed for markers of oxidative stress and untargeted metabolomics. Additionally, the impact of sustained fructose intake on colonic microbiota was also evaluated. High fructose intake for 10 weeks decreased protein digestibility and promoted changes in the physiological digestion of proteins, enhancing intestinal digestion rather than stomach digestion. Moreover, at colonic stages, the oxidative stress was harmfully increased, and both the microbiota and the intraluminal colonic metabolome were modified.
Keyphrases
- oxidative stress
- protein protein
- healthcare
- public health
- mass spectrometry
- mental health
- diabetic rats
- binding protein
- small molecule
- anaerobic digestion
- health information
- ischemia reperfusion injury
- high resolution
- induced apoptosis
- signaling pathway
- physical activity
- endothelial cells
- high glucose
- human health
- weight loss
- heat shock protein