Cytotoxicity of Halogenated Tyrosyl Compounds, an Emerging Class of Disinfection Byproducts.
Dayong TianBirget MoeGuang HuangPing JiangZong-Chao LingXing-Fang LiPublished in: Chemical research in toxicology (2020)
Halogenated amino acids and peptides are an emerging class of disinfection byproducts (DBPs), having been detected in drinking water and in washed food products. However, the toxicological significance of these emerging DBPs remains unclear. In this study, the cytotoxicity of eight halogenated tyrosyl compounds was investigated in Chinese hamster ovary (CHO) cells using real-time cell analysis (RTCA). Dihalogenated tyrosyl compounds are more cytotoxic than their monohalogenated analogues. The cytotoxicity of the dihalogenated compounds is associated with their ability to induce intracellular reactive oxygen species (ROS), suggesting that oxidative stress is an important toxicity pathway of these compounds. Pearson correlation analysis of the cytotoxicity (IC50 values) of these compounds with eight physicochemical parameters showed strong associations with their lipophilicity (logP) and reactivity (polarizability, ELUMO). Finally, cytotoxicity testing of the concentrated extracts of a chloraminated mixture of eight dipeptides with bromide or iodide showed the cytotoxicity of these mixtures in the order: iodinated peptides > brominated peptides ≥ chlorinated peptides. These results demonstrate that halogenated peptide DBPs are toxicologically relevant, and further research is needed to understand the implications of long-term exposure for human health.
Keyphrases
- drinking water
- reactive oxygen species
- human health
- amino acid
- oxidative stress
- health risk assessment
- health risk
- risk assessment
- induced apoptosis
- climate change
- stem cells
- cell death
- ionic liquid
- mesenchymal stem cells
- signaling pathway
- heavy metals
- cell proliferation
- bone marrow
- mass spectrometry
- anti inflammatory
- pi k akt
- high speed