Exposure of Drosophila melanogaster to Mancozeb Induces Oxidative Damage and Modulates Nrf2 and HSP70/83.
Miriane Acosta SaraivaEduardo da Rosa ÁvilaGustavo Felipe da SilvaGiulianna Echeverria MacedoNathane Rosa RodriguesPatrícia de Brum VieiraMariele Samuel NascimentoRochele Sogari PicolotoIllana Kemmerich MartinsNelson Rodrigues de CarvalhoJeferson Luis FrancoThaís PosserPublished in: Oxidative medicine and cellular longevity (2018)
Mancozeb (MZ), a manganese- and zinc-containing ethylene-bis-dithiocarbamate, is a broad-spectrum fungicide. Harmful effects of this fungicide have been reported in nontarget organisms via a not fully understood mechanism. Drosophila melanogaster has provided remarkable contributions for toxicological studies. This work was aimed at evaluating the biochemical targets and implication of oxidative stress in MZ-mediated toxicity in drosophilas. Exposure of flies for fifteen days to MZ at 5 and 10 mg/mL through the diet impaired locomotor performance and induced fly mortality. In parallel, it caused lipid peroxidation and reactive oxygen species (ROS) formation and Mn overload. MZ inhibited superoxide dismutase and inducted catalase and glutathione S-transferase activities. Nitric oxide and reduced glutathione levels were significantly decreased by MZ. Heat shock proteins (HSP70 and HSP83) and Nrf2 mRNA levels were significantly augmented in MZ-exposed flies. Our study reinforced the use of Drosophila melanogaster as a reliable model for the study of biochemical targets of pesticides, and based on our data, MZ induced oxidative damage and Mn accumulation in a concentration-dependent manner. An adaptative cellular state was inducted by the lower concentration of pesticide, possibly contributing to the slighter damage observed.
Keyphrases
- drosophila melanogaster
- heat shock
- oxidative stress
- diabetic rats
- heat shock protein
- reactive oxygen species
- heat stress
- nitric oxide
- dna damage
- risk assessment
- physical activity
- ischemia reperfusion injury
- induced apoptosis
- oxide nanoparticles
- electronic health record
- drug induced
- cell death
- machine learning
- room temperature
- cardiovascular events
- weight loss
- ionic liquid
- nitric oxide synthase
- binding protein
- high resolution
- mass spectrometry
- artificial intelligence
- gas chromatography
- tissue engineering
- case control
- endothelial cells