Differential effects of biocompatible peritoneal dialysis fluids on human mesothelial and endothelial cells in 2D and 3D phenotypes.
Rajesh M JagirdarEleanna PitarakiErasmia RoukaEleftherios D PapazoglouMaria BartosovaPantelis ZebekakisClaus Peter SchmittSotirios G ZarogiannisVassilios LiakopoulosPublished in: Artificial organs (2023)
In 3D phenotypes the effects of PDF are more uniform in both mesothelial and endothelial cells, mitigating spheroid formation and gel contraction. On the contrary, effects on 2D phenotypes are more uniform in the icodextrin and amino acid PDF as opposed to glucose ones and affect mesothelial cells more variably. 2D and 3D comparative assessments of PDF effects on the main peritoneal membrane cell barriers, the mesothelial and endothelial, could provide useful translational information for PD studies.
Keyphrases
- endothelial cells
- high glucose
- peritoneal dialysis
- end stage renal disease
- amino acid
- induced apoptosis
- vascular endothelial growth factor
- single cell
- chronic kidney disease
- oxidative stress
- drug delivery
- cell proliferation
- mesenchymal stem cells
- ionic liquid
- metabolic syndrome
- cell death
- endoplasmic reticulum stress
- case control
- hyaluronic acid
- social media