Quantum Dots for Monitoring Choline Consumption Process of Living Cells via an Electrostatic Force-Mediated Energy Transfer.

In this work, a ratiometric nanoprobe CdS/ZnS-FB was designed for H 2 O 2 detection based on FRET assay. Furthermore, CdS/ZnS-FB could work for detecting choline (Ch) and acetylcholine (ACh) since H 2 O 2 is the enzyme cascade reaction product. Significantly, the Jurkat T's choline consumption could also be quantitatively measured by monitoring FRET ratio ( I 522 / I 426 ). Thus, the biosensor could be applied as a universal tool for the detection of choline consumption of living cells, which provides a good potential for the applications in detecting chemical transmitter and cancer diagnosis.