Rapid and visual detection of Staphylococcus aureus in milk using a recombinase polymerase amplification-lateral flow assay combined with immunomagnetic separation.
Ya-Lei WangXin ZhangQuan WangPeng-Xuan LiuWei TangRong GuoHai-Yang ZhangZhao-Guo ChenXian-Gan HanWei JiangPublished in: Journal of applied microbiology (2022)
The newly developed IMS-lateral flow RPA-LF assay effectively combines sample preparation, amplification and detection into a single platform. Because of its high sensitivity, specificity and speed, the IMS-RPA-LF assay will have important implications for the rapid detection of S. aureus in contaminated food.