DNA and protein-generated chimeric molecules for delivery of influenza viral epitopes in mouse and humanized NSG transfer models.
Nikolina M MihaylovaIliyan K ManoylovMaria H NikolovaJozsef PrechlAndrey Ivanov TchorbanovPublished in: Human vaccines & immunotherapeutics (2024)
Purified subunit viral antigens are weakly immunogenic and stimulate only the antibody but not the T cell-mediated immune response. An alternative approach to inducing protective immunity with small viral peptides may be the targeting of viral epitopes to immunocompetent cells by DNA and protein-engineered vaccines. This review will focus on DNA and protein-generated chimeric molecules carrying engineered fragments specific for activating cell surface co-receptors for inducing protective antiviral immunity. Adjuvanted protein-based vaccine or DNA constructs encoding simultaneously T - and B-cell peptide epitopes from influenza viral hemagglutinin, and scFvs specific for costimulatory immune cell receptors may induce a significant increase of anti-influenza antibody levels and strong CTL activity against virus-infected cells in a manner that mimics the natural infection. Here we summarize the development of several DNA and protein chimeric constructs carrying influenza virus HA317-41 fragment. The generated engineered molecules were used for immunization in intact murine and experimentally humanized NSG mouse models.
Keyphrases
- circulating tumor
- sars cov
- cell free
- single molecule
- immune response
- amino acid
- protein protein
- induced apoptosis
- cell therapy
- cell cycle arrest
- binding protein
- signaling pathway
- cell surface
- nucleic acid
- mouse model
- dendritic cells
- oxidative stress
- small molecule
- endoplasmic reticulum stress
- bone marrow
- cancer therapy