Immunohistochemical markers for histopathological diagnosis and differentiation of acute cutaneous graft-versus-host disease.
Joanna WegnerBeate Weidenthaler-BarthJulia EngelbertMax KnotheClaudia BraunDoris HelbigChristopher SacherAndreas KreftEva M WagnerMirjana ZiemerRalf G MeyerEsther von StebutPublished in: Experimental dermatology (2021)
Graft-versus-host disease (GvHD) is a major complication following stem-cell or solid-organ transplantation. Accurate diagnosis of cutaneous GvHD is challenging, given that drug eruptions and viral rashes may present with similar clinical/histological manifestations. Specific markers are not available. We performed the histological examination of biopsy samples from acute GvHD (aGvHD; n = 54), Stevens-Johnson syndrome/toxic epidermal necrolysis (SJS/TEN; n = 27), maculopapular drug eruption (MDE; n = 26) and healthy controls (n = 26). Samples of aGvHD showed a decrease in Langerhans cells (LC, p = 0.0001) and an increase in macrophages (MΦ, p = 0.0001) compared to healthy skin. Compared to SJS/TEN, MDE and healthy skin, aGvHD biopsies contained greater numbers of CD4+ and CD8+ T cells. The majority of CD4+ T-helper cells were localized in the upper dermis, whereas cytotoxic CD8+ T cells were found in the epidermis. Increased numbers of CD56+ natural killer (NK) cells in the upper dermis of aGvHD skin (p = 0.007) were not observed in controls or SJS/TEN and MDE. There were no differences in elafin staining between aGvHD and the latter two conditions. Acute GvHD appears to have a distinct inflammatory cell profile (T cells/NK cells) that may aid establishing in a more accurate diagnosis, especially when used to rule out differential diagnoses such as SJS/TEN or MDE.
Keyphrases
- nk cells
- liver failure
- induced apoptosis
- drug induced
- respiratory failure
- allogeneic hematopoietic stem cell transplantation
- stem cells
- wound healing
- cell cycle arrest
- soft tissue
- aortic dissection
- cell therapy
- oxidative stress
- sars cov
- dendritic cells
- ultrasound guided
- signaling pathway
- immune response
- acute myeloid leukemia
- extracorporeal membrane oxygenation
- cell death
- tandem mass spectrometry
- liquid chromatography
- bone marrow
- acute respiratory distress syndrome