Ferrocene-Doped Polystyrene Nanoenzyme and DNAzyme Cocatalytic SERS Quantitative Assay of Ultratrace Pb 2 .
Chongning LiZhenghong WangZhiliang JiangPublished in: Nanomaterials (Basel, Switzerland) (2022)
A new, stable and high-catalytic activity ferrocene-doped polystyrene nanosphere (PN Fer ) sol was prepared by the hydrogel procedure and characterized by electron microscopy and molecular spectroscopy. Results show that the nanosol exhibits excellent catalysis of the new indicator nanoreaction between AgNO 3 and sodium formate to generate nanosilver with strong surface-enhanced Raman scattering (SERS), resonance Rayleigh scattering (RRS) and surface plasmon resonance absorption (Abs) trimode molecular spectral signals. This new nanocatalytic amplification trimode indicator reaction was coupled with the G-quadruplex DNAzyme catalytic amplification of Pb 2+ aptamer to fabricate a new SERS quantitative/RRS/Abs assay platform for the determination of ultratrace amounts of Pb 2+ . The Pb 2+ content in water samples was analyzed with satisfactory results.
Keyphrases
- label free
- heavy metals
- sensitive detection
- gold nanoparticles
- quantum dots
- high throughput
- high resolution
- aqueous solution
- electron microscopy
- raman spectroscopy
- single molecule
- nucleic acid
- risk assessment
- drug delivery
- highly efficient
- minimally invasive
- energy transfer
- optical coherence tomography
- molecularly imprinted
- solid state
- hyaluronic acid
- liquid chromatography
- fluorescent probe