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Photocaged 5-(Hydroxymethyl)pyrimidine Nucleoside Phosphoramidites for Specific Photoactivatable Epigenetic Labeling of DNA.

Aswathi ChakrapaniViola Vaňková HausnerováOlatz Ruiz-LarrabeitiRadek PohlLibor KrásnýMichal Hocek
Published in: Organic letters (2020)
5-Hydroxymethylcytosine and uracil are epigenetic nucleobases, but their biological roles are still unclear. We present the synthesis of 2-nitrobenzyl photocaged 5-hydroxymethyl-2'-deoxycytidine and uridine 3'-O-phosphoramidites and their use in automated solid-phase synthesis of oligonucleotides (ONs) modified at specific positions. The ONs were used as primers for PCR to construct DNA templates modified in the promoter region that allowed switching of transcription through photochemical uncaging.
Keyphrases
  • dna methylation
  • circulating tumor
  • gene expression
  • cell free
  • single molecule
  • transcription factor
  • nucleic acid
  • machine learning
  • high throughput
  • real time pcr