Combining intramuscular and intranasal homologous prime-boost with a chimpanzee adenovirus-based COVID-19 vaccine elicits potent humoral and cellular immune responses in mice.
Xingxing LiLing WangJingjing LiuEnyue FangXiaohui LiuQinhua PengZelun ZhangMiao LiXinyu LiuXiaohong WuDanhua ZhaoLihong YangJia LiShouchun CaoYanqiu HuangLeitai ShiHongshan XuYunpeng WangYue SuoGuangzhi YueJianhui NieWei Jin HuangWenjuan LiYuhua LiPublished in: Emerging microbes & infections (2022)
The efficacy of many coronavirus disease 2019 (COVID-19) vaccines has been shown to decrease to varying extents against new severe acute respiratory syndrome coronavirus 2 variants, which are responsible for the continuing COVID-19 pandemic. Combining intramuscular and intranasal vaccination routes is a promising approach for achieving more potent immune responses. We evaluated the immunogenicity of prime-boost protocols with a chimpanzee adenovirus serotype 68 vector-based vaccine, ChAdTS-S, administered via both intranasal and intramuscular routes in BALB/c mice. Intramuscular priming followed by an intranasal booster elicited the highest levels of IgG, IgA, and pseudovirus neutralizing antibody titres among all the protocols tested at day 42 after prime immunization compared with the intranasal priming/intramuscular booster and prime-boost protocols using only one route. In addition, intramuscular priming followed by an intranasal booster induced high T-cell responses, measured using the IFN-γ ELISpot assay, that were similar to those observed upon intramuscular vaccination. All ChAdTS-S vaccination groups induced Th1-skewing of the T-cell response according to intracellular cytokine staining and Meso Scale Discovery cytokine profiling assays on day 56 after priming. This study provides reference data for assessing vaccination schemes of adenovirus-based COVID-19 vaccines with high immune efficacy.
Keyphrases
- coronavirus disease
- respiratory syndrome coronavirus
- immune response
- sars cov
- high throughput
- high glucose
- small molecule
- diabetic rats
- dengue virus
- oxidative stress
- metabolic syndrome
- escherichia coli
- dna damage
- drug induced
- zika virus
- machine learning
- gene expression
- big data
- anti inflammatory
- insulin resistance
- endothelial cells