Applications of the Methylotrophic Yeast Komagataella phaffii in the Context of Modern Biotechnology.
Lidia Maria Pepe de MoraesHenrique Fetzner MarquesViviane Castelo Branco ReisCíntia Marques CoelhoMatheus de Castro LeitãoAlexsandro Sobreira GaldinoThais Paiva Porto de SouzaLuiza Cesca PivaAna Laura Alfonso PerezDébora TrichezJoão Ricardo Moreira de AlmeidaJanice Lisboa De MarcoFernando Araripe Gonçalves TorresPublished in: Journal of fungi (Basel, Switzerland) (2024)
Komagataella phaffii (formerly Pichia pastoris ) is a methylotrophic yeast widely used in laboratories around the world to produce recombinant proteins. Given its advantageous features, it has also gained much interest in the context of modern biotechnology. In this review, we present the utilization of K. phaffii as a platform to produce several products of economic interest such as biopharmaceuticals, renewable chemicals, fuels, biomaterials, and food/feed products. Finally, we present synthetic biology approaches currently used for strain engineering, aiming at the production of new bioproducts.