Development of in situ hybridization for detection of elephant endotheliotropic herpesvirus in Asian elephants.
Varankpicha KochakulKittikorn BoonsriSaruda TiwananthagornChalermchart SomgirdChatchote ThitaramKidsadagon PringproaPublished in: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc (2018)
Elephant endotheliotropic herpesvirus (EEHV) is one of the most important viral infectious diseases affecting the elephant population worldwide, especially juveniles and young adults. We developed a chromogenic in situ hybridization (ISH) test for detection of EEHV in Asian elephants ( Elephas maximus). Digoxigenin (DIG) DNA probes from the polymerase and terminase genes of EEHV were synthesized using a PCR DIG-labeling method, and detection of hybridized probe to target EEHV DNA was carried out by anti-DIG immunolabeling. Distribution of EEHV-1A and EEHV-4 genomes was found to be prominent in mononuclear phagocytic cells of spleen and endothelial cells of visceral organs. ISH enables the detection of EEHV infection and has applications in understanding pathogenesis of EEHV in Asian elephants.
Keyphrases
- real time pcr
- loop mediated isothermal amplification
- young adults
- label free
- endothelial cells
- infectious diseases
- single molecule
- induced apoptosis
- circulating tumor
- type diabetes
- living cells
- gene expression
- cell free
- sars cov
- genome wide
- photodynamic therapy
- metabolic syndrome
- dna methylation
- cell proliferation
- transcription factor
- sensitive detection
- cell cycle arrest
- skeletal muscle
- cell death
- circulating tumor cells