Linear Peptides Containing d-Leucine with Neuroprotective Activities from the Leech Whitmania pigra Whitman.
Zi-Yue ZhangNan MaLi-Jun TaoXue-Ying GongWen-Cai YeLei WangPublished in: Journal of natural products (2019)
Three new linear peptides containing d-leucine, named whitmantides A-C (1-3), were isolated from the dried whole bodies of Whitmania pigra Whitman. Their structures with absolute configurations were elucidated by Edman degradation, mass spectrometry, Marfey's analysis, and solid-phase synthesis. It is the first time that peptides containing d-amino acid in leeches were discovered. Compounds 1-3 displayed neuroprotective activities against oxygen-glucose deprivation/reperfusion injury on Neuro-2a cells. In addition, ex vivo serum stability tests showed that 1-3 were resistant to protease degradation.
Keyphrases
- amino acid
- cerebral ischemia
- mass spectrometry
- induced apoptosis
- high resolution
- subarachnoid hemorrhage
- cell cycle arrest
- brain injury
- liquid chromatography
- blood brain barrier
- acute myocardial infarction
- blood glucose
- blood pressure
- oxidative stress
- acute ischemic stroke
- high performance liquid chromatography
- skeletal muscle
- type diabetes
- capillary electrophoresis
- coronary artery disease
- adipose tissue
- cell death
- weight loss
- pi k akt
- acute coronary syndrome
- percutaneous coronary intervention
- cell proliferation