Accuracy of PCR universal primer for methicillin-resistant Staphylococcus and comparison of different phenotypic screening assays.

Dayanne A de MeloBianca da S SoaresCássia C da MottaFelipe C DubenczuckNicolle L BarbieriCatherine M LogueShana de O CoelhoIrene da S CoelhoMiliane Moreira Soares de Souza

Published in: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2019)

Characterization of methicillin-resistant Staphylococcus (MRS) is a continuous challenge at diagnostic laboratories. The phenotypic methods present heterogeneous results and the occurrence of variants of mecA gene turned this goal even more challenging to achieve. The present study provided an accurate and highly discriminatory screening tool for MRS, improving its detection.

Keyphrases

staphylococcus aureus
biofilm formation
copy number
methicillin resistant staphylococcus aureus
real time pcr
risk assessment
genome wide
gene expression
dna methylation
label free