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Quantifying the Impact of Nasopharyngeal Specimen Quality on Severe Acute Respiratory Syndrome Coronavirus 2 Test Performance.

Melissa Richard-GreenblattMatthew J ZieglerValerie BrombergElizabeth HuangHatem AbdallahPam TolomeoEbbing LautenbachLaurel GlaserBrendan J Kelly
Published in: Open forum infectious diseases (2021)
Variability in NP specimen quality significantly impacts the performance of clinical SARS-CoV-2 assays, and caution should be taken when interpreting quantitative SARS-CoV-2 Ct results. If unrecognized, low-quality NP specimens, which are characterized by a low level of amplifiable human DNA target, may limit the successful application of SARS-CoV-2 Ct values to direct infection control and public health interventions.
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