Fusobacterium nucleatum Interaction with Pseudomonas aeruginosa Induces Biofilm-Associated Antibiotic Tolerance via Fusobacterium Adhesin A.
Qian LiLisi TanHongyan WangYurong KouXiaoting ShiShuwei ZhangYaping PanPublished in: ACS infectious diseases (2020)
Respiratory infections with Pseudomonas aeruginosa or Fusobacterium nucleatum are associated with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) and failure in antibiotic treatment. However, the impact of these dual-species interactions on the severity of chronic obstructive pulmonary disease (COPD) and biofilm antibiotic susceptibility remains poorly understood. This study demonstrated that F. nucleatum frequently coexisted with P. aeruginosa in the respiratory tract, and the number of F. nucleatum was negatively correlated with the lung function of AECOPD patients. The coculture of P. aeruginosa and F. nucleatum promoted bacterial proliferation and induced antibiotic tolerance through the formation of a dense biofilm surrounded by excessive Pel and Psl polysaccharides. Moreover, Fusobacterium adhesin A (FadA), rather than F. nucleatum spent medium, induced antibiotic tolerance of the P. aeruginosa biofilm. These results indicate that F. nucleatum is a biomarker of lung function decline in AECOPD patients and interacts with P. aeruginosa in vitro to resist antibiotics via FadA, which would be a potential anti-infective target of these dual-species infection.
Keyphrases
- pseudomonas aeruginosa
- lung function
- cystic fibrosis
- chronic obstructive pulmonary disease
- biofilm formation
- end stage renal disease
- staphylococcus aureus
- ejection fraction
- candida albicans
- respiratory tract
- air pollution
- acinetobacter baumannii
- newly diagnosed
- prognostic factors
- peritoneal dialysis
- drug induced
- signaling pathway
- high glucose
- liver failure
- intensive care unit
- climate change
- drug resistant
- endothelial cells
- genetic diversity
- weight loss
- binding protein
- mechanical ventilation
- body mass index
- respiratory failure
- aortic dissection
- acute respiratory distress syndrome
- patient reported