Mechanism Analysis of Antiangiogenic d-Isofloridoside from Marine Edible Red algae Laurencia undulata in HUVEC and HT1080 cell.
Shengtao YangZhenbang XiaoLiyuan LinYanfei TangPengzhi HongShengli SunChunxia ZhouZhong-Ji QianPublished in: Journal of agricultural and food chemistry (2021)
Laurencia undulata, as one of the most biologically active species in the genus Laurencia, is an edible folk herb red algae. Among them, d-isofloridoside (DIF, 940.68 Da) is isolated from Laurencia undulata, which has antioxidant and matrix metalloproteinases (MMP) inhibitory activities. However, its mechanism of action on tumor angiogenesis has not yet been reported. In this study, we have studied the mechanism of DIF on tumor metastasis and angiogenesis in HT1080 cell and human vascular endothelial cell (HUVEC). The results show that DIF can reduce the activity of MMP-2/9, and can inhibit the expression of hypoxia-inducible factor-1α (HIF-1α) by regulating the downstream PI3K/AKT and mitogen-activated protein kinases (MAPK) pathways, thereby down-regulating the production of vascular endothelial growth factor (VEGF) in CoCl2-induced HT1080 cell. In addition, DIF can inhibit the activation of VEGF receptor (VEGFR-2), regulate downstream PI3K/AKT, MAPK, nuclear factor-kappa B (NF-κB) signal pathways, activate apoptosis, and thus down-regulate the production of platelet-derived growth factor (PDGF) in VEGF-induced HUVEC. In conclusion, our research shows that DIF has the potential to develop into a tumor-preventing functional food and tumor angiogenesis inhibitor, and it can provide theoretical guidance for the high-value comprehensive utilization of edible red algae Laurencia undulata.
Keyphrases
- vascular endothelial growth factor
- pi k akt
- endothelial cells
- high glucose
- signaling pathway
- cell cycle arrest
- nuclear factor
- growth factor
- cell proliferation
- single cell
- oxidative stress
- toll like receptor
- cell therapy
- long non coding rna
- anti inflammatory
- diabetic rats
- mesenchymal stem cells
- risk assessment
- climate change
- bone marrow
- immune response
- cell migration
- binding protein