Application of Nucleic Acid-Based Strategies to Detect Infectious Pathogens in Orthopaedic Implant-Related Infection.
Emily Ann McClurePaul M WerthBenjamin D RossIda Leah GitajnPublished in: The Journal of bone and joint surgery. American volume (2023)
The best practices for interpreting nucleic acid-based diagnostic techniques include considering positive and negative controls, reads per sample, detection thresholds (for differentiating contaminants from positive results), and the primer set or targeted regions.