Dengue Virus-Polymersome Hybrid Nanovesicles for Advanced Drug Screening Using Real-Time Single Nanoparticle-Virus Tracking.
Hyun-Ouk KimWoonsung NaMinjoo YeomJong-Woo LimEun-Hye BaeGeunseon ParkChaewon ParkHwunjae LeeHye Kwon KimDae Gwin JeongKwang-Soo LyooVan Phan LeSeungjoo HaamDae-Sub SongPublished in: ACS applied materials & interfaces (2020)
Dengue virus (DENV) is a major infectious viral pathogen that affects millions of individuals worldwide every year, causing a potentially fatal syndrome, while no commercial antiviral drugs are yet available. To develop an antiviral against dengue fever, it is necessary to understand the relationship between DENV and host cells, which could provide a basis for viral dynamics and identification of inhibitory drug targets. In this study, we designed DiD-loaded and BODIPY-ceramide-encapsulated DENV-polymersome hybrid nanovesicles (DENVSomes) prepared by an extrusion method, which trigger red fluorescence in the endosome and green in the Golgi. DENVSome monitors the dynamics of host cell-virus interaction and tracking in living cells with novel state-of-the-art imaging technologies that show images at high resolution. Also, DENVSome can be exploited to screen whether candidate antiviral drugs interact with DENVs. Consequently, we successfully demonstrated that DENVSome is an efficient tool for tracking and unraveling the mechanisms of replication and drug screening for antiviral drugs of DENV.
Keyphrases
- dengue virus
- zika virus
- living cells
- high resolution
- aedes aegypti
- fluorescent probe
- single molecule
- drug induced
- sars cov
- induced apoptosis
- drug delivery
- cell cycle arrest
- optical coherence tomography
- stem cells
- adverse drug
- high throughput
- cell proliferation
- convolutional neural network
- cell therapy
- emergency department
- mass spectrometry
- oxidative stress
- case report
- machine learning
- endoplasmic reticulum stress
- signaling pathway
- candida albicans
- pi k akt
- iron oxide