An enzyme-controlled mesoporous nanomachine for triple-responsive delivery.
Beatriz MayolVictor DatoManuel RodriguezElena LucenaAnabel VillalongaPaula DíezSandra Jimenez-FalcaoFélix SancenónAlfredo SánchezDiana VilelaPaloma Martínez-RuizRamón Martínez-MáñezReynaldo VillalongaPublished in: Journal of materials chemistry. B (2022)
The construction of a novel enzyme-controlled nanomachine with multiple release mechanisms for on-command delivery is described. This nanodevice was assembled by modifying mesoporous silica nanoparticles with 2-(benzo[ d ]thiazol-2-yl)phenyl 4-aminobenzoate moieties, and further capped with β-cyclodextrin-modified glucose oxidase neoglycoenzyme. The device released the encapsulated payload in the presence of H 2 O 2 and acidic media. The use of glucose as an input chemical signal also triggered cargo release through the enzymatic production of gluconic acid and hydrogen peroxide, and the subsequent disruption of the gating mechanism at the mesoporous surface. The nanodevice was successfully employed for the enzyme-controlled release of doxorubicin in HeLa cancer cells.