A Workflow for the Enrichment, the Identification, and the Isolation of Non-Apoptotic Single Circulating Tumor Cells for RNA Sequencing Analysis.

Anna AbramovaMahdi RivandiLiwen YangNadia StammJan-Philipp CieslikEllen HonischDieter NiederacherTanja FehmTanja N Fehm Andre Franken

Published in: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2023)

This workflow enables the enrichment, detection, and isolation of single CTCs for individual transcriptome analyses. The discrimination of apoptotic and non-apoptotic cells allows to focus on CTCs with a high RNA integrity to ensure a successful transcriptome analysis. This article is protected by copyright. All rights reserved.

Keyphrases

circulating tumor cells
cell death
cell cycle arrest
single cell
anti inflammatory
induced apoptosis
circulating tumor
electronic health record
rna seq
gene expression
loop mediated isothermal amplification
label free
data analysis
nucleic acid