A Versatile Platform for Mass Spectrometry Imaging of Arbitrary Spatial Patterns.
Kenneth P GarrardMåns EkelöfSitora KhodjaniyazovaM Caleb BagleyDavid C MuddimanPublished in: Journal of the American Society for Mass Spectrometry (2020)
A vision-system driven platform, RastirX, has been constructed for mass spectrometry imaging (MSI) of arbitrary two-dimensional patterns. The user identifies a region of interest (ROI) by drawing on a live video image of the sample with the computer mouse. Motion commands are automatically generated to move the sample to acquire scan data for the pixels in the ROI. Synchronization of sample stage motion with laser firing and mass spectrometer (MS) scan acquisition is fully automated. RastirX saves a co-registered optical image and the scan location information needed to convert raw MS data into imzML format. Imaging an arbitrarily shaped ROI instead of the minimal enclosing rectangle reduces contamination from off-sample material and significantly reduces acquisition time.
Keyphrases
- high resolution
- mass spectrometry
- deep learning
- high speed
- computed tomography
- liquid chromatography
- high throughput
- multiple sclerosis
- high performance liquid chromatography
- gas chromatography
- capillary electrophoresis
- machine learning
- ms ms
- electronic health record
- big data
- risk assessment
- magnetic resonance imaging
- gene expression
- tandem mass spectrometry
- healthcare
- drinking water
- heavy metals
- photodynamic therapy
- contrast enhanced