Supercritical-Fluid Chromatography On-Chip with Two-Photon-Excited-Fluorescence Detection for High-Speed Chiral Separations.
Josef J HeilandDavid GeisslerSebastian K PiendlRico WariasDetlev BelderPublished in: Analytical chemistry (2019)
Herein, we present the first example of microchip-based supercritical-fluid chromatography (SFC). A microfluidic-glass-chip platform with pressure and temperature control for fast and efficient on-column injection is described. This enabled fast and efficient separation of chiral and achiral compounds within seconds and also employed two-photon-excitated-fluorescence detection. Peak shapes were highly regular and symmetric even for linear flow rates over the packed microchip column in a range of up to 20 mm·s-1.
Keyphrases
- high speed
- capillary electrophoresis
- liquid chromatography
- mass spectrometry
- high throughput
- atomic force microscopy
- circulating tumor cells
- label free
- tandem mass spectrometry
- energy transfer
- high resolution
- single molecule
- loop mediated isothermal amplification
- real time pcr
- living cells
- high performance liquid chromatography
- ionic liquid
- single cell
- solid phase extraction
- ms ms
- sensitive detection
- fluorescent probe