3‴- O -Foliamenthoyl-Rutin, a New Flavonoid Glycoside from the Roots of Nymphoides peltata .

Nymphoides peltata (Menyanthaceae) has been used as a medicinal herb in traditional medicines to treat conditions such as strangury, polyuria, swelling, and as a diuretic and antipyretic. In our ongoing research to discover novel structural and/or biological natural products in natural resources, five flavonoids, quercetin ( 1 ), quercitrin ( 2 ), isoquercetin ( 3 ), quercetin-3- O -vicianoside ( 4 ), and rutin ( 5 ), as well as a new flavonoid glycoside, 3‴- O -foliamenthoyl-rutin ( 6 ), were isolated from the MeOH extract of N. peltata roots. The chemical structure of the new compound ( 6 ) was determined by analyzing 1D and 2D NMR spectra and high-resolution (HR) electrospray ionization mass spectroscopy (ESIMS), along with a chemical reaction. The wound-healing activities of the isolated compounds ( 1 - 6 ) were evaluated using a HaCaT cell scratch test. Among the isolates, isoquercetin ( 3 ), quercetin-3- O -vicianoside ( 4 ), and 3‴- O -foliamenthoyl-rutin ( 6 ) promoted HaCaT cell migration over scratch wounds, with compound 4 being the most effective. Our findings provide experimental data supporting the potential of quercetin-3- O -vicianoside ( 4 ) as a wound-healing agent.