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Synthesis of fusidane triterpenoid Mannich bases as potential antibacterial and antitumor agents.

Elena V SalimovaLyudmila V ParfenovaDiana V IshmetovaLiana F ZainullinaYulia V Vakhitova
Published in: Natural product research (2023)
Mannich bases (8 examples) were synthesized via aminomethylation of fusidane propargyl esters. In vitro antimicrobial screening against key ESKAPE pathogens showed that the fusidic acid based Mannich products exhibit a high antimicrobial effect against Gram-positive bacteria Staphylococcus aureus and the fungus Cryptococcus neoformans . Moreover, the cytotoxic effect of fusidic acid and its analogs, which showed high antibacterial activity, was determined by MTT assay on cancer HepG2, HCT-116, SH-SY5Y, MCF-7, A549 and conditionally normal cells HEK293. A remarkable cytotoxic activity of fusidic acid propargyl ester and its aminomethylene derivatives against cancer and nontumoral HEK293 cells with IC 50 values within 4.2-25 µM was found.
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