Quadruplex DNA-Stabilising Dinuclear Platinum(II) Terpyridine Complexes with Flexible Linkers.
Dale L AngBenjamin W J HarperLeticia CuboOscar MendozaRamon VilarJanice Rae Aldrich-WrightPublished in: Chemistry (Weinheim an der Bergstrasse, Germany) (2015)
Four dinuclear terpyridineplatinum(II) (Pt-terpy) complexes were investigated for interactions with G-quadruplex DNA (QDNA) and duplex DNA (dsDNA) by synchrotron radiation circular dichroism (SRCD), fluorescent intercalator displacement (FID) assays and fluorescence resonance energy transfer (FRET) melting studies. Additionally, computational docking studies were undertaken to provide insight into potential binding modes for these complexes. The complexes demonstrated the ability to increase the melting temperature of various QDNA motifs by up to 17 °C and maintain this in up to a 600-fold excess of dsDNA. This study demonstrates that dinuclear Pt-terpy complexes stabilise QDNA and have a high degree of selectivity for QDNA over dsDNA.
Keyphrases
- energy transfer
- single molecule
- quantum dots
- circulating tumor
- high resolution
- living cells
- atomic force microscopy
- high throughput
- molecular dynamics simulations
- molecular dynamics
- radiation therapy
- risk assessment
- dna binding
- case control
- radiation induced
- circulating tumor cells
- transcription factor
- protein protein