Microfluidic epigenomic mapping technologies for precision medicine.
Chengyu DengLynette B NalerChang LuPublished in: Lab on a chip (2019)
Epigenomic mapping of tissue samples generates critical insights into genome-wide regulations of gene activities and expressions during normal development and disease processes. Epigenomic profiling using a low number of cells produced by patient and mouse samples presents new challenges to biotechnologists. In this review, we first discuss the rationale and premise behind profiling epigenomes for precision medicine. We then examine the existing literature on applying microfluidics to facilitate low-input and high-throughput epigenomic profiling, with emphasis on technologies enabling interfacing with next-generation sequencing. We detail assays on studies of histone modifications, DNA methylation, 3D chromatin structures and non-coding RNAs. Finally, we discuss what the future may hold in terms of method development and translational potential.
Keyphrases
- genome wide
- dna methylation
- high throughput
- single cell
- copy number
- high resolution
- gene expression
- induced apoptosis
- systematic review
- clinical trial
- dna damage
- cell cycle arrest
- case report
- high density
- current status
- signaling pathway
- transcription factor
- circulating tumor cells
- climate change
- oxidative stress
- case control
- cell proliferation
- endoplasmic reticulum stress
- label free