Metastatic colorectal cancer treatment response evaluation by ultra-deep sequencing of cell-free DNA and matched white blood cells.
Iris van 't ErveJamie E MedinaAlessandro LealEniko PappJillian A PhallenVilmos AdleffElaine Jiayuee ChiaoAdith S ArunKaren BolhuisJohn K SimmonsAanavi KarandikarKenneth C ValkenburgMark SausenSamuel V AngiuoliRobert B ScharpfCornelis J A PuntGerrit A MeijerVictor E VelculescuRemond J A FijnemanPublished in: Clinical cancer research : an official journal of the American Association for Cancer Research (2022)
Accurate calling of ctDNA mutations for treatment response monitoring is feasible in a tumor tissue-independent manner by combined cfDNA and patient-matched WBC genomic DNA analysis. This tissue biopsy-independent approach simplifies sample logistics and facilitates the application of liquid biopsy ctDNA-testing for evaluation of emerging therapy resistance, opening new avenues for early adaptation of treatment regimens.
Keyphrases
- circulating tumor
- metastatic colorectal cancer
- ultrasound guided
- induced apoptosis
- high resolution
- fine needle aspiration
- cell free
- circulating tumor cells
- cell cycle arrest
- case report
- single molecule
- single cell
- stem cells
- copy number
- ionic liquid
- oxidative stress
- gene expression
- cell death
- replacement therapy
- nucleic acid