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Purification of crime scene DNA extracts using centrifugal filter devices.

Lina NorénRonny HedellRicky AnsellJohannes Hedman
Published in: Investigative genetics (2013)
Amicon Ultra 30 K performed better than Microsep 30 K due to higher DNA recovery and more efficient removal of PCR-inhibitory substances. The different performances of the filter devices are likely caused by the quality of the filters and plastic wares, for example, their DNA binding properties. DNA purification using centrifugal filter devices can be necessary for successful DNA profiling of impure crime scene samples and for consistency between different PCR-based analysis systems, such as quantification and STR analysis. In order to maximize the possibility to obtain complete STR DNA profiles and to create an efficient workflow, the level of DNA purification applied should be correlated to the inhibitor-tolerance of the STR analysis system used.
Keyphrases
  • circulating tumor
  • cell free
  • single molecule
  • dna binding
  • nucleic acid
  • transcription factor
  • high resolution
  • single cell